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Extracting DNA Using Phenol-Chloroform - PacBio

Page 1 Please note: the shared protocols described herein may not have been validated by Pacific Biosciences and are provided as-is and without any warranty. Use of these protocols is offered to those customers who understand and accept the associated terms and conditions and wish to take advantage of their potential to help prepare samples for analysis Using the PacBio RS system. If any of these protocols are to be used in a production environment, it i s the responsibility of the end user to perform the required validation. Extracting DNA Using Phenol-Chloroform Reagents Needed 1. phenol / chloroform /isoamyl alcohol (PCI) solution (25:24:1) DNase (RNase- and Protease-Free - Molecular Biology grade), pH Important: acidic pH makes the DNA go into the phenolic layer while RNA goes into the aqueous layer Suppliers 2. chloroform /isoamyl alcohol, 24:1 (Molecular Biology grade) Suppliers 3.

Extracting DNA Using Phenol-Chloroform . Reagents Needed . 1. Phenol/chloroform/isoamyl a lcohol (PCI) solution (25:24:1) DNase (RNase- and Protease-Free - Molecular Biology grade), pH 7.8-8.2 Important: acidic pH makes the DNA go into the phenolic layer while RNA goes into the aqueous layer Suppliers

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For Research Use Only. Not for use in diagnostic procedures.

www.pacb.com

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Procedure & Checklist – Preparing Multiplexed Microbial ...

www.pacb.com

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Procedure & Checklist – Using AMPure PB Beads for Size ...

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