Transcription of Förster resonance energy transfer
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F rster resonance energy transfer1F rster resonance energy transferJablonski diagram of FRET with typicaltimescales rster ( fluorescence ) resonance energy transfer (FRET), resonance energy transfer (RET) or electronic energy transfer (EET), is a mechanism describing energy transfer between twochromophores.[1] A donor chromophore, initially in its electronicexcited state, may transfer energy to an acceptor chromophore throughnonradiative dipole dipole coupling.[2] The efficiency of this energytransfer is inversely proportional to the sixth power of the distancebetween donor and acceptor making FRET extremely sensitive to smalldistances.
In fluorescence microscopy, fluorescence confocal laser scanning microscopy, as well as in molecular biology, FRET is a useful tool to quantify molecular dynamics in biophysics and biochemistry, such as protein-protein interactions, protein–DNA interactions, and protein conformational changes. For monitoring the complex formation
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