Factors that Affect Enzyme Catalysis

1 Factors that Affect Enzyme CatalysisIonic strength: = (Zi2ci)Review: urea, guanidiniumhydrochloride, detergents, or organic solventsTemperaturepHFactors that Affect Enzyme Catalysis : Ionic strength: = (Zi2ci)NaCl (mM) (107M-1s-1) with O2-for active Cu2+Cu+2+ O2- Cu++ O2Cu++ O2-+ 2 H+ Cu+2+ H2O2 Superoxide Dismutase2 Conditions that cause protein denaturation: of urea orguanidiniumhydrochloride (G@@HCl)-Urea and G@@HClboth H-bond to proteins causingdisruption of the H-bond unfold and remain soluble (usually). denaturation renaturation Folded proteinC O 2-N H 3+RRRU nfolded proteinC O 2-RRRN H 3+COH2 NNH2 CNH2+H2 NNH2Cl-Conditions that cause protein denaturation: Soaps/Detergents denaturation renaturation Folded proteinC O 2-N H 3+RRRU nfolded proteinC O 2-RRRN H 3+Addition of soaps or detergentsNonpolarportions of soaps and detergents interact with protein R groups causing the loss of the hydrophobic denature and remain structures formed insodium dodecylsulfate (SDS)C O 2-RRRN H 3+3 Conditions that cause protein denaturation: Organic Solvents denaturationprotein precipitateswith poorly definedstructure renaturation see belowC O 2-RRRN H 3+C O 2-RRN H 3+RFolded proteinC O

1 Factors that Affect Enzyme Catalysis Ionic strength: m = ½ S(Z i 2c i) Review: urea, guanidinium hydrochloride, detergents, or organic solvents Temperature

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