Transcription of Flash Column Chromatography Guide - MIT OpenCourseWare
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Flash Column Chromatography Guide - MIT OpenCourseWare
- Flash Column Chromatography Guide Overview: Flash Column Chromatography is a quick and (usually) easy way to separate complex mixtures of compounds. We will be performing relatively large scale separations in , around g of compound. Columns are often smaller in scale than this and some of you will experience these once you move into the research lab. Column Chromatography uses the same principles discussed in the TLC Handout, but can be used on a preparative scale. We are running Flash columns since we will use compressed air to push the solvent through the Column . This not only helps provide better separation, but also cuts down the amount of time required to run a Column .
elution. This simply means that you increase the polarity of the solvent running through the column (eluent) throughout the course of the purification. This technique will be described in more detail later in the handout, but for the TLC analysis you should determine which solvent systems put the different spots in the 0.2 to 0.3 Rf range. ...
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