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Fluorescence Spectroscopy: A tool for Protein folding ...

1 Fluorescence spectroscopy : a tool for Protein folding /unfolding Study PC3267 Updated in Jan. 2007 2 introduction Principle of Fluorescence Fluorescence is the result of a three-stage process that occurs in certain molecules called fluorophores or fluorescent dyes. A fluorescent probe is a fluorophore designed to localize within a specific region of a biological specimen or to respond to a specific stimulus. The process responsible for the Fluorescence of fluorescent probes and other fluorophores is illustrated by the simple electronic-state diagram (Jablonski diagram) shown in Figure 1.

2 Introduction Principle of fluorescence Fluorescence is the result of a three-stage process that occurs in certain molecules called fluorophores or fluorescent dyes.

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