Transcription of for FLUORESCENCE MICROSCOPY - Chroma
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for FLUORESCENCE MICROSCOPY - Chroma
Chroma TECHNOLOGY CORPHANDBOOK ofOPTICAL FILTERSfor FLUORESCENCEMICROSCOPYby JAY REICHMAN 2017AN INTRODUCTION TOFLUORESCENCE MICROSCOPY 2 Excitation and emission spectraBrightness of the FLUORESCENCE signalThe FLUORESCENCE microscopeTypes of filters used in FLUORESCENCE microscopyThe evolution of the FLUORESCENCE microscopeA GENERAL DISCUSSION OF OPTICAL FILTERS 8 TerminologyAvailable products Colored filter glass Thin-film coatings Acousto-optical filters Liquid Crystal Tunable FiltersDESIGNING FILTERS FORFLUORESCENCE MICROSCOPY 14 Image Contrast FLUORESCENCE spectra Light sources Detectors BeamsplittersOptical quality Optical quality parameters Optical quality requirements for wide-field microscopyFILTER SETS FOR SUB-PIXEL REGISTRATION 24 FILTERS FOR confocal MICROSCOPY 25 Optical quality requirements Nipkow-disk scanning Laser scanningSpectral requirements Nipkow-disk scanning Laser scanningFILTERS FOR MULTIPLE PROBE APPLICATIONS 29 REFERENCES 30 GLOSSARY 31 Chroma TECHNOLOGY CORPHANDBOOK ofOPTICAL FILTERSfor FLUORESCENCEMICROSCOPYby JAY REICHMAN 1 FLUORESCENCE MICROSCOPY requ
microscopes and applications, including wide-field microscopes, confocal microscopes, and applications involving simultaneous detection of multiple fluorescent probes. Also included is information on the terms used to describe and specify optical filters and practical information on how filters can affect the optical alignment of a microscope.
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