Transcription of FortéBio Bio-layer Interferometry Kinetic Analysis Tutorial
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FortéBio Bio-layer Interferometry Kinetic Analysis Tutorial
Selvakumar Dakshnamurthy, PhD Richard Yip, PhDField Applications ScientistsFort BioBio-layer Interferometry Kinetic Analysis TutorialCONFIDENTIAL2 Introduction to Biolayer Interferometry (s. 3-12) Kinetic Analysis Basics (s. 13-18) Basic kinetics Data Acquisition (s. 19-35) Data Analysis HT (s. 36-63 )OutlineCONFIDENTIAL3 Full life-cycle offering for biomolecular interaction Analysis Label-free assays based on Bio-layer Interferometry (BLI) and Surface Plasmon Resonance (SPR) platforms Instruments, consumables, software, post-sale services (one-on-one training)ForteBio is a Market Leader in Label-Free Biomolecular AnalysisOctet & BLItzPioneerBio-Layer Interferometry (BLI)SPRCONFIDENTIAL4 Octet RED96eOctet K2 Octet QKeOctet RED384 Octet HTXM olecular Weight Range> 150 Da> 150 Da> 5000 Da> 150 Da> 150 Da# Spectrometers8211616# Channels per Read828161 -96 MicroplatePositions11122 Biosensor RerackingYesYesYesYesYesRobot CompatibleNoNoNoYesYesSample Vessel Formats969696, 96 HA96 / 96HA 384 / 384TW96 / 96HA 384 / 384 TWMinimum Sample Volume180 L per well180 L per well180 L per well40 L per well40 L per wellAffinity range (approximate)
Kinetic Analysis Tutorial. 2 CONFIDENTIAL • Introduction to Biolayer Interferometry (s. 3-12) • Kinetic Analysis Basics (s. 13-18) ... • The two reflected beams interfere constructively or destructively at the spectrometer CCD detector array Ref 1 2 ... the temperature from 2 °C above ambient up to 40 °C, to allow the instrument ...
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