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Frequencies & Percentages - Stemcell Technologies

1. Cell type Frequencies and Percentages are for 6 to 12 week old C57BL/6 or C57BL/6J mice. The values will vary depending on mouse strain, age, gender, and environment. 2. Data sourced from: A. The Jackson Laboratory. Flow-cytometric analysis of 11 inbred strains of mice. MPD:Jaxpheno6. Mouse Phenome Database website, The Jackson Laboratory, Bar Harbor, Maine, USA. , Aug, 2013; B. The Jackson Laboratory. Hematological survey of 11 inbred strains of mice. MPD:Jaxpheno4. Mouse Phenome Database website, The Jackson Laboratory, Bar Harbor, Maine, USA. , Aug, 2013. 3. Data sourced from: C. Stemcell Technologies RND Scientists. 4. Peripheral blood values expressed as cells / L of blood. Spleen and bone marrow values expressed as % of total leukocytes. 5. EasySep /RoboSep Immunomagnetic Isolation. 6. Negative selection labels unwanted cells to isolate desired untouched cells . 7. Positive selection labels and isolates desired cell types.

Hematopoietic Progenitor Cells Bone Marrow 0.17 ± 0.03% 19856 18757 (CD117+) 18756 (Sca1+) 18856 (Sca1+) ... EasySep™ is a powerful immunomagnetic cell isolation platform that combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic

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Frequencies Cell Types Human Peripheral Blood

www.stemcell.com

3. Remove erythrocytes from whole blood samples with EasySep™ RBC Depletion Reagent or EasySep™ Red Blood Cell Lysis Buffer. 4. Use HetaSep™ or Ammonium Chloride Solution to obtain nucleated cells from blood samples. HetaSep™ is an erythrocyte aggregation agent used to quickly separate nucleated cells from red blood cells (RBCs) in ...

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