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Fundamentals of UV-Visible Spectroscopy (5965-5123E)

: Page i Friday, December 20, 1996 4:12 PM. Contents Chapter 1 Principles and applications of UV-Visible Spectroscopy Basic 10. The electromagnetic 10. Wavelength and frequency .. 11. Origin of UV-Visible spectra .. 11. Transmittance and absorbance .. 14. Derivative 14. Obtaining derivative spectra .. 16. applications .. 17. Signal-to-noise .. 17. Instrumental considerations .. 18. Qualitative 18. Identification spectra and structure .. 18. Confirmation of identity .. 19. 21. Other qualitative information .. 22. Protein and nucleic acid melting 22. Enzyme activity .. 23. Instrumental 24. Quantitative 24. Beer's 24. Sample 28. Multicomponent analysis .. 29. Principle of additivity .. 29. Simple simultaneous equations 29. Least squares 32. Other 34. Sample 34. Instrumental requirements .. 34. Indirect quantification .. 35. Chemical 35. Spectrophotometric titrations .. 35. Enzyme kinetic 36. Chapter 2 Instrumentation Instrumental 38. 38. 39. Dispersion devices .. 40. i : Page ii Friday, December 20, 1996 4:12 PM.

Principles and applications of UV-visible spectroscopy The energy associated with electromagnetic radiation is defined by the following equation: where E is energy (in joules), h is Planck’s constant (6.62 × 10-34Js), and ν is frequency (in seconds). Wavelength and frequency Electromagnetic radiation can be considered a combination of

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