Transcription of Genotyping By Sequencing (GBS) Method Overview
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Genotyping By Sequencing (GBS) Method Overview
Genotyping By Sequencing (GBS) Method OverviewRJ Elshire, JC Glaubitz, Q Sun, JV HarrimanES Buckler, and SE Background/Goals GBS lab protocol Illumina Sequencing review GBS adapter system How GBS differs from RAD Modifying GBS for different species Workflow in our labTopics PresentedBackgroundGenotyping by Sequencing (GBS) in any large genome species requires reduction of genome Restriction Enzymes (REs)I. Target enrichment Long range PCR of specific genes or genomic subsets Molecular inversion probes Sequence capture approaches hybridization based (microarrays) *Technically less challenging* Methylation sensitive REs filter out repetitive genomic fractionQTL are often located in non coding regionsVgt1, Tb, B regulatory regions 60 150kb from geneExon Exon ExonExonExon captureMap large numbers ofgenome wide markersQTLC reate inexpensive, robust multiplex Sequencing protocolLow coverageSequencing Illumina GAInformatics PipelinAnchor markers across the genomeImpute missing dataCombine genotypic & phenotypic data for GS and GWASOur goal is to create a public Genotyping /informatics platform based on next generation sequencingOpen Source Method available for anyone to use / modify.
Background Genotyping by sequencing (GBS) in any large genome species requires reduction of genome complexity. I. Target enrichment II.
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