Glucose UV Reagent - Cliniqa

33024_01 11/23/09 2008 Cliniqa Corporation. All rights reserved. Glucose UV Reagent Order No. Description R80017 10 x 20 mL R80036 10 x 50 mL R85411 4 x 1000 mL INTENDED USE For the quantitative in vitro enzymatic determination of Glucose in biological fluids. TEST SUMMARY The method for determination of Glucose employing the Hexokinase (HK) Glucose -6-phosphate dehydrogenase (G-6-P-DH) reactions was developed by G. Bucolo for Calbiochem in 1961 and was marketed by the latter Company in 1962 (1). The method is highly specific for Glucose ; the Reagent can be used for the determination of Glucose in serum, plasma, urine and cerebrospinal fluid without prior treatment of these specimens. Common anticoagulants, including fluoride, at the recommended levels, do not interfere. The assay is based on the following reactions: HK Glucose + ATP G-6-P + ADP G-6-P-DH G-6-P + NAD 6-PG + NADH Glucose is phosphorylated by adenosine-5'-triphosphate (ATP), in a reaction catalyzed by HK.

33024_01 11/23/09 © 2008 Cliniqa Corporation. All rights reserved. ® Glucose UV Reagent Order No. Description R80017 10 x 20 mL R80036 10 x 50 mL

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