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Guide to Performing Relative Quantitation of Gene ...
Guide to Performing Relative Quantitation of Gene Expression Using real - time Quantitative PCR 1 Guide to Performing Relative Quantitation of Gene Expression Using real - time Quantitative PCR Table of Contents Section I: Introduction to real - time PCR and Relative Quantitation of Gene Expression pg 4 1. Introduction 2. What is Relative Quantitation ? 3. Terms and Acronyms 4. Relative Quantitation of Gene Expression Requires the Quantitation of Two Different Genes (Target and Endogenous Control) 5. Factors Affecting Accurate real - time PCR Results 6. What is PCR Amplification Efficiency? Section II: RNA Preparation and Reverse Transcription pg 14 1. Introduction 2. Quantifying Input RNA 3. Reverse Transcription (RT) for Relative Quantitation of Gene Expression a. Two-step RT-PCR b. One-step RT-PCR 4. Selecting Reverse Transcription and real - time PCR Reagents 5.
Real-Time PCR System • Applied Biosystems StepOnePlus™ Real-Time PCR System • Applied Biosystems 7300 Real-Time PCR System • Applied Biosystems 7500 Real-Time PCR System • Applied Biosystems 7500 Fast Real-Time PCR System • Applied Biosystems 7900HT FAST Real-Time PCR System • ABI® PRISM 7000 Sequence Detection System 4
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