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Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR 1 Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR Table of Contents Section I: Introduction to Real-Time PCR and Relative Quantitation of Gene Expression pg 4 1. Introduction 2. What is Relative Quantitation ? 3. Terms and Acronyms 4. Relative Quantitation of Gene Expression Requires the Quantitation of Two Different Genes (Target and Endogenous Control) 5. Factors Affecting Accurate Real-Time PCR Results 6. What is PCR Amplification Efficiency? Section II: RNA Preparation and Reverse Transcription pg 14 1.
The rate at which a PCR amplicon is generated, commonly measured as a percentage value. If a particular PCR amplicon doubles in ... examine the effect of drug treatment on the level of a mouse mRNA, ... • Monitoring: Replicates can be used to monitor the precision of the
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