Guide to Solid Phase Extraction - Sigma-Aldrich

Bulletin 910T197910 1998 Sigma-Aldrich Phase Extraction (SPE) is an increasingly useful sample preparation technique. With SPE, many of the problems associated withliquid/liquid Extraction can be prevented, such as incomplete Phase separations, less-than-quantitative recoveries, use of expensive,breakable specialty glassware, and disposal of large quantities of organic solvents. SPE is more efficient than liquid/liquid Extraction ,yields quantitative extractions that are easy to perform, is rapid, and can be automated. Solvent use and lab time are is used most often to prepare liquid samples and extract semivolatile or nonvolatile analytes, but also can be used with solidsthat are pre-extracted into solvents. SPE products are excellent for sample Extraction , concentration, and cleanup. They are availablein a wide variety of chemistries, adsorbents, and sizes. Selecting the most suitable product for each application and sample is Types2 Reversed Phase packingsNormal Phase packingsIon exchange packingsAdsorption packingsSPE Theory3 How compounds areretained by the sorbentReversed Phase SPEN ormal Phase SPEIon exchange SPES econdary interactionsThe role of pH in SPEHow to Use SPE6 Selecting the properextraction schemeThe five-step SPE methoddevelopment processSample pretreatment options- Liquid samples- Solid samplesSPE hardware and accessoriesfor processing samples9960028 Guide to Solid Phase Extraction9950124997031299402569960029 SUPELCOB ulletin 9102sSPE tu

Bulletin 910 T197910 ©1998 Sigma-Aldrich Co. Introduction Solid phase extraction (SPE) is an increasingly useful sample preparation technique. With SPE, many of the problems associated with

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