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Homework Solutions for Chem 422

Homework Solutions for Chem 422 Chapter )Elution is the process of washing substances through a chromatographiccolumn with the mobile )The mobile phase in chromatography is the one that moves over orthrough an immobilized phase in a column or on the surface of a )The stationary phase in a chromatographic column is a solid or liquid thatis fixed in )The distribution constant (K) in chromatography is the ratio of theconcentration of the analyte in the stationary phase to its concentration inthe mobile phase when the two phases are in )The retention time (tR) for an analyte is the time between its injection ontoa column and the appearance of its peak as it elutes from the )The retention factor (k) is the ratio of the amount of analyte in thestationary phase to the amount in the mobile phase. It is generallycalculated by k = (tR - tM)/tM = tR )The selectivity factor ( ) of a column for two analytes (A eluting before B)is given by = KB/KA = k (B)/k (A) = tR (B)/tR (A).

Homework Solutions for Chem 422 Chapter 26 1. a) Elution is the process of washing substances through a chromatographic column with the mobile phase. b) The mobile phase in chromatography is the one that moves over or through an immobilized phase in a column or on the surface of a plate.

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