Transcription of HPLC METHOD DEVELOPMENT AND VALIDATION: AN …
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HPLC METHOD DEVELOPMENT AND VALIDATION: AN …
274 Santhosh al. / Vol 4 / Issue 4 / 2014 / 274-280. HPLC METHOD DEVELOPMENT AND VALIDATION: AN OVERVIEW G. Santhosh*, , , Maheswara Rao Department of Pharmaceutical Analysis and Quality Assurance, CMR College of Pharmacy, Medchal Road, Kandlaykoya, Hyderabad, India. ABSTRACT High performance liquid chromatography (HPLC) is an analytical technique which is proficient to separate, detect and quantify various drugs and its related degradants. An appropriate mobile phase, column, column temperature, wavelength and gradient must be found that affords suitable compatibility and stability of drug as well as degradants and impurities.
Keywords: Method validation, Method development, High PressureLiquid Chromatography (HPLC). INTRODUCTION HPLC is an analytical technique in which solutes are resolved by differential rates of elution as they pass through a chromatographic column. The method of separation by this instrument is governed by distribution
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