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HPLC METHOD DEVELOPMENT AND VALIDATION: AN …

274 Santhosh al. / Vol 4 / Issue 4 / 2014 / 274-280. HPLC METHOD DEVELOPMENT AND VALIDATION: AN OVERVIEW G. Santhosh*, , , Maheswara Rao Department of Pharmaceutical Analysis and Quality Assurance, CMR College of Pharmacy, Medchal Road, Kandlaykoya, Hyderabad, India. ABSTRACT High performance liquid chromatography (HPLC) is an analytical technique which is proficient to separate, detect and quantify various drugs and its related degradants. An appropriate mobile phase, column, column temperature, wavelength and gradient must be found that affords suitable compatibility and stability of drug as well as degradants and impurities.

International Journal of Pharmaceutical Research & Analysis e-ISSN: 2249 – 7781 Print ISSN: 2249 – 779X ... analyte and a number of generic runs may be processed in ... International Union of Pure and Applied Chemistry (IUPAC) have expressed the view that “Specificity is the ...

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