Transcription of HPLC Troubleshooting Guide
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HPLC Troubleshooting Guide
HPLC Troubleshooting Guide Peak Tailing HPLC Columns John Dolan Your decision has lasting effects. Choose wisely. HPLC Columns Ultra Inert Base-Deactivated HPLC Columns For Performance, Selectivity and Guaranteed Reproducibility . performance ACE guarantee If ACE does not outperform your existing column (of equivalent phase, particle size and dimensions), send in your comparative data within 60. days and keep the ACE column FREE OF CHARGE. HPLC Troubleshooting Guide Contents Page Troubleshooting Tables 2. Peak Shape 2.
3.1. Late elution 16 3.2. Ghost peaks in gradient runs 17 3.3. Negative peaks 17 4. Column Backpressure 18 4.1. Locating pressure problems 18 4.2. In-line filters 18 4.3. Guard cartridges 18 4.4. Buffer precipitation 19 5. Column Care 20 5.1. Equilibration 20 5.2. Column flushing 20 5.3. Column storage 22 6. Summary 22 7. References 23 8 ...
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