Transcription of HPLC Troubleshooting Guide
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HPLC Troubleshooting Guide
HPLC Troubleshooting Guide Peak Tailing HPLC Columns John Dolan Your decision has lasting effects. Choose wisely. HPLC Columns Ultra Inert Base-Deactivated HPLC Columns For Performance, Selectivity and Guaranteed Reproducibility . performance ACE guarantee If ACE does not outperform your existing column (of equivalent phase, particle size and dimensions), send in your comparative data within 60. days and keep the ACE column FREE OF CHARGE. HPLC Troubleshooting Guide Contents Page Troubleshooting Tables 2. Peak Shape 2. Retention Variation 3. Ghost Peaks 4. Column Backpressure 5. Introduction 6. 1. Peak Shape 6. Peak tailing 6.
2. Add basic mobile phase additive (eg. TEA) – not needed with ultra-high purity phases Chelation with metal ions in stationary phase As above Wrong mobile phase pH 1. Decrease mobile phase pH to suppress silanol ionisation (see 1.2) 2. Increase buffer concentration (see 1.2) Blocked frit 1. Reverse flush the column (see 5.2) 2.
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