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Hydrolysis Cyclic 3':5'-Adenosine Monophosphate ...

, , ,June1974 Short-TermBindingandHydrolysisofCyclic3' :5'-AdenosineMonophosphatebyAggregatingD ictyosteliumCells(chemotaxis/receptors/c yclic-AMPphosphodiesterase/cellmembranes /slimemolds) ,74 Tabingen,GermanyCommunicatedbySaulRosema n,April3, , ,kinetics, (cAMP)actsasanattractantforaggregatingce llsoftheslimemold,Dictyosteliumdiscoideu m(1,2), (3).ExtracellularcAMPishydrolyzedinthiso rganismbothbyextracellularandcell-boundp hosphodiesterases,thelatterexhibitingits highestactivityatthestageofaggrega-tion- competence(4,5).CyclicGMP(cGMP)isagoodsu bstrateforthesephos-phodiesterasesandapo tentinhibitorofcAMPhydrolysisbytheseenzy mes(6);itschemotacticandotherbiologicala ctivities,however,are1000-foldlowerthant hoseofcAMP(2,3).Theseresultssuggestthatt hereceptorsitesaremorespecificthanthecat alyticsitesofphosphodiesterase,andprompt edstudiesofcAMPbindingunderconditionsofp hosphodiesteraseinhibitioninordertodetec treceptorsthat,inlivingcells, (7).

Cyclic AMP(cAMP) acts as an attractant for aggregating cells of theslime mold,Dictyostelium discoideum (1, 2), imply- ingthatthese cells …

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  Mapc, 5 adenosine monophosphate, Adenosine, Monophosphate

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