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Immunocytochemistry (ICC) - Novus Biologicals

Learn more | more | (ICC) HandbookConjugateExcitation/EmissionEmis sionColorLaser (Excitation Source)NotesDyLightTM 405400/420 VioletViolet (405 nm)Bright and photostableAlexa Fluor 405401/421 VioletViolet (405 nm)Best when used with more abundant targetsDyLightTM 350353/432 Violet-BlueUltraviolet (355 nm)Can be used with DyLightTM 488, 594 and 647 in multiplexingAlexa Fluor 350346/442 Violet-BlueUltraviolet (355 nm)Often used with Alexa Fluor 488, 594 and 647 in multiplexing, best for high-abundance targetsDyLightTM 488493/518 GreenBlue (488) Brighter, photostable replacement for FITC; not suitable for use with GFPA lexa Fluor 488495/519 GreenBlue (488) Photostable over a broad pH range; replaces FITCFITC495/519 GreenBlue (488) Small organic fluorophore; cannot be used with DyLightTM 488, Alexa Fluor 488 or GFPDyLightTM 405LS397/572 YellowViolet (405 nm)Superior alternative to Pacific Orange; good choice for multicolor applications on the violet laserAlexa Fluor 546556/573 YellowYellow-Green (561 nm)Photostable over a broad pH range; brighter than Cy3 DyLightTM 550562/576 YellowYellow-Green (561 nm)

Fluorescence quantum yield is a measurement of emission efficiency and is calculated by the formula: ΦF = number of emitted photons/number of absorbed photons. The maximum quantum yield possible (ΦF = 1) signifies a 100% efficient fluorescence process. An example of a fluorochrome with high quantum yield is Alexa Fluor® 488 which has ΦF= 0.92.

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