Incorporation by MMLV-Derived Reverse …

Base Preferences in Non- templated NucleotideIncorporation by MMLV-Derived Reverse TranscriptasesPawel Zajac a, Saiful Islam, Hannah Hochgerner b, Peter L nnerberg, Sten Linnarsson*Laboratory for Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, SwedenAbstractReverse transcriptases derived from Moloney Murine Leukemia Virus (MMLV) have an intrinsic terminal transferaseactivity, which causes the addition of a few non- templated nucleotides at the 3 end of cDNA, with a preference forcytosine. This mechanism can be exploited to make the Reverse transcriptase switch template from the RNAmolecule to a secondary oligonucleotide during first-strand cDNA synthesis, and thereby to introduce arbitrarybarcode or adaptor sequences in the cDNA. Because the mechanism is relatively efficient and occurs in a singlereaction, it has recently found use in several protocols for single-cell RNA sequencing.

Base Preferences in Non-Templated Nucleotide Incorporation by MMLV-Derived Reverse Transcriptases Pawel Zajac¤a, Saiful Islam, Hannah Hochgerner¤b, Peter Lönnerberg, Sten Linnarsson* Laboratory for Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden

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  Reserve, Preference, Nucleotide, Templated, Preferences in non templated nucleotide, Reverse transcriptases, Transcriptases

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