Transcription of Insulinum humanum - uspbpep.com
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Insulinum humanum - uspbpep.com
Insulin, human EUROPEAN PHARMACOPOEIA 01/2005:0838 B. Peptide mapping ( ). SELECTIVE CLEAVAGE OF THE PEPTIDE BONDS. INSULIN, HUMAN Test solution. Prepare a mg/ml solution of the substance to be examined in M hydrochloric acid Insulinum humanum and transfer 500 l of this solution to a clean tube. Add ml of HEPES buffer solution pH R and 400 l of a 1 mg/ml solution of Staphylococcus aureus strain V8. protease R. Cap the tube and incubate at 25 C for 6 h. Stop the reaction by adding ml of sulphate buffer solution pH R. Reference solution. Prepare at the same time and in the same manner as for the test solution but using human insulin CRS instead of the substance to be examined.
Insulin injection, biphasic EUROPEAN PHARMACOPOEIA 5.0 Test solution. Dissolve 40.0 mg of the substance to be examined in 0.01 M hydrochloric acid and dilute to …
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