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Interpreting Nanodrop (Spectrophotometric) Results

Interpreting Nanodrop ( spectrophotometric ) Results Foundation of Spectrophotometry: The Beer Lambert Law what does it mean? A = cl Where A=absorbance, =extinction coefficient, c=concentration and l=path length. The Beer Lambert law draws a direct correlation between absorbance and concentration. While nucleic acids absorb at many wavelengths, they have a peak absorbance of UV light at 260nm. Thus, the amount of light absorbed in this region can be used to determine the concentration of RNA or DNA in solution by applying the Beer Lambert law.

difficult to assess the purity of the sample by analyzing the A260/280 and A260/230 ratios. This sample may actually be good, but it cannot be assessed by the NanoDrop because it is outside the lowest concentration the NanoDrop is designed to measure. A more sensitive method such

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