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Introduction to Biotechnology

BIOL1414 Lab Manual Fall 2011 1 Austin Community College, Biotechnology Department Introduction to Biotechnology Fall 2011 Linnea Fletcher, Evelyn Goss, Patricia Phelps, Angela Wheeler, and Shelley O Grady ISBN: BIOL1414F2011 BIOL 1414 Laboratory Manual BIOL1414 Lab Manual Fall 2011 2 BIOL1414 Lab Manual Fall 2011 3 Table of Contents LAB UNIT 1: Introduction TO BIOL1414 LAB .. 5 Lab Unit 1-A: Documentation: The Lab Notebook & Lab Report .. 11 Lab Unit 1-B: Biotechnology Laboratory Security & Safety .. 17 Lab Unit 1-C: Lab Equipment & Reagent Orientation .. 25 Lab Unit 1-D: Math Skills for the Laboratory .. 31 LAB UNIT 2: CURRENT GOOD MANUFACTURING PRACTICES (cGMP) .. 39 LAB UNIT 3: BASIC TOOLS IN THE Biotechnology LABORATORY .. 85 Lab Unit 3-A: Using a 91 Lab Unit 3-B: Calibrating Lab Instruments .. 97 LAB UNIT 4: PREPARING SOLUTIONS .. 103 LAB UNIT 5: TOTAL RNA ISOLATION FROM ALFALFA SPROUTS .. 115 Lab Unit 5-A: Total RNA Isolation .. 121 Lab Unit 5-B: Denaturing Gel Electrophoresis of Total RNA.

Introduction to Biotechnology – Course Competencies The ACC Biotechnology Program has been designed to match the needs of the biotechnology job market in our immediate area. We have invited industrial partners from our community to contribute to the competency goals for each course, including this introductory course, to assure

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