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Introduction to Biotechnology

BIOL1414 Lab Manual Fall 2011 1 Austin Community College, Biotechnology Department Introduction to Biotechnology Fall 2011 Linnea Fletcher, Evelyn Goss, Patricia Phelps, Angela Wheeler, and Shelley O Grady ISBN: BIOL1414F2011 BIOL 1414 laboratory Manual BIOL1414 Lab Manual Fall 2011 2 BIOL1414 Lab Manual Fall 2011 3 Table of Contents LAB UNIT 1: Introduction TO BIOL1414 LAB .. 5 Lab Unit 1-A: Documentation: The Lab Notebook & Lab Report .. 11 Lab Unit 1-B: Biotechnology laboratory Security & Safety .. 17 Lab Unit 1-C: Lab Equipment & Reagent Orientation .. 25 Lab Unit 1-D: Math Skills for the laboratory .. 31 LAB UNIT 2: CURRENT GOOD MANUFACTURING PRACTICES (cGMP) .. 39 LAB UNIT 3: BASIC TOOLS IN THE Biotechnology laboratory .. 85 Lab Unit 3-A: Using a 91 Lab Unit 3-B: Calibrating Lab Instruments .. 97 LAB UNIT 4: PREPARING SOLUTIONS .. 103 LAB UNIT 5: TOTAL RNA ISOLATION FROM ALFALFA SPROUTS.

LAB UNIT 1: INTRODUCTION TO BIOL1414 LAB Welcome to your first course in biotechnology! This course will emphasize its laboratory component to reflect the importance of your training in biotechnology skills. Keep in mind as you work your way through this manual the specific purposes in each exercise. They will

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