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Introduction to Confocal Laser Scanning Microscopy (LEICA)

Introduction to Confocal Laser Scanning Microscopy (LEICA). This presentation has been put together as a common effort of Urs Ziegler, Anne Greet Bittermann, Mathias Hoechli. Many pages are copied from Internet web pages or from presentations given by Leica, Zeiss and other companies. Please browse the internet to learn interactively all about optics. For questions &. registration please contact . Confocal Laser Scanning Microscopy xy yz 100 m xz 100 m xy yz xz thick specimens at different depth 3D reconstruction Types of Confocal microscopes {. {. {. point Confocal slit Confocal spinning disc Confocal (Nipkov). Best resolution and out-of-focus suppression as well as highest multispectral flexibility is achieved only by the classical single point Confocal system ! Fundamental Set-up of fluorescence Microscopes: Confocal vs.}}}

Fluorescence Microscopy. Confocal laser scanning microscope - set up: The system is composed of a a regular ßorescence microscope and the confocal part, including scan head, laser optics, computer. Higher z-resolution and reduced out-of-focus-blur make confocal pictures crisper

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  Fluorescence, Microscopy, Fluorescence microscopy

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