Transcription of Introduction to Fluorescence and Phosphorescence
{{id}} {{{paragraph}}}
1 Introduction to Fluorescence and Phosphorescence Credits: This notes are a summary from Modern Analytical Chemistry by David Harvey, Molecular Fluorescence : Principles and Applications (Wiley 2001) by Bernard Valeur, and application notes from Perkin Elmer Instruments. Prepared by Jose Hodak for 2302303 Spectroscopy for Chemistry BSAC program 2008. Photoluminescence in the ultraviolet-visible comprises two similar phenomena: Fluorescence and Phosphorescence . Molecules have energy levels determined by the molecular orbitals that hold the molecule bound together. In the case of atoms it is the atomic orbitals what determines the energy levels of the electrons. In this section we will concern ourselves with molecular photoluminescence. We will leave the atomic emission phenomena for the next section. Absorption of an ultraviolet or visible photon promotes a valence electron from its ground state to an excited state with conservation of the electron s spin.
Introduction to Fluorescence and Phosphorescence Credits: This notes are a summary from “Modern Analytical Chemistry” by David Harvey, “Molecular Fluorescence: Principles and Applications” (Wiley 2001) by Bernard Valeur, and application notes from Perkin Elmer Instruments.
Domain:
Source:
Link to this page:
Please notify us if you found a problem with this document:
{{id}} {{{paragraph}}}
Introduction to Fluorescence, INTRODUCTION, Fluorescence Microscopy, Introduction to Fluorescence Microscopy, FLUORESCENCE, Fluorescence Imaging, Introduction Fluorescence, Lecture Notes: Introduction to Spectroscopy, INTRODUCTION TO ENERGY-DISPERSIVE X, Introduction Fluorescence Microscopy, X-Ray Fluorescence