Transcription of Ion exchange columns and media - Hebrew …
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Ion exchange columns and media - Hebrew …
GE Healthcare Ion exchange columns and media Selection Guide imagination at work General information Principle of Ion exchange equilibration sample injection gradient elution wash re-equilibration Chromatography volume high salt wash 1M. 5 CV. Ion exchange (IEX) chromatography can separate molecules tightly bound molecules or groups of molecules that have only slight differences in unbound molecules elute elute in high salt wash charge. Separation is based on the reversible interaction before gradient begins between a charged molecule and an oppositely charged [NaCl]. chromatography medium. Typically, conditions are selected to ensure that the molecules of interest bind to the medium 10 20 CV. as they are loaded onto the column. Conditions are then 5 10 CV. 5 10 CV. altered so that the bound substances are eluted differentially. 0. Column volumes [CV]. Elution is most often performed by a continuous gradient Fig 1. Typical IEX separation using gradient elution. or a stepwise increase in ionic strength, most commonly using NaCl.
GE Healthcare Ion exchange columns and media Selection Guide imagination at work
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