Transcription of Ion fragmentation of small molecules in mass spectrometry
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Ion fragmentation of small molecules in mass spectrometry
1J. Prasain 01/19/11 Ion fragmentation of small molecules in mass spectrometryJeevan Prasain 01/19/11 Nomenclature: the main names and acronyms used in mass spectrometry Molecular ion:Ion formed by addition or the removal of oneor several electrons to or from the sample molecules -Electron Impact (EI-MS). M + e- M+ + 2e- Adduct Ion:Ion formed through interaction of two speciesand containing all the atoms of one of them plus one orseveral atoms of them ( alkali, ammonium).2J. Prasain 01/19 Pseudomolecular ion:Ion originating from the analyte molecule by abstraction of a proton [M-H]-or addition of proton [M+H]+ Tandem mass spectrometry (Cooks, 1976): MS/MS (McLafferty, 1978), tandem in space or time Precursor ion/parent ion:Ions undergoing fragmentation .
• Adduct Ion: Ion formed through ... Concentration, uM 0.0 2.0e4 4.0e4 6.0e4 8.0e4 1.0e5 1.2e5 1.4e5 1.6e5 1.8e5 2.0e5 2.2e5 2.4e5 2.6e5 2.8e5 3.0e5 s Sensitivity is an issue in quantification of steroids. 10 J. Prasain 01/19/11 Derivatization of estradiol with dansyl chloride leads to the formation of E 2-dansyl (m/z 506) Source: Nelson et ...
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