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KOD -Plus-

2 mM dNTPs 1.0 ml × 1 polymerase antibodies that neutralize polymerase and 3’J5’ exonuclease activity. Quality check can be performed by amplifying the β-globin gene (3.6 Kb) and p53 gene Total Mutant KOD -Plus- 10,610 10 0.09 High fidelity PCR enzyme (A company) 10,900 68 0.62 Pfu based DNA polymerase 6,520 76 1.17

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www.agilent.com

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assets.thermofisher.com

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KAPA HiFi PCR Kit Kapa/ Kit Codes and Components

rochesequencingstore.com

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rochesequencingstore.com

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Polymerase Chain Reaction (PCR)

www.eeescience.utoledo.edu

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(2–5μL) 101– 5 cells/2μL medium or PBS μL 1–2 × 1 mm As the concentration of extracted cm 1 –2 DNA is low, 35 40 cycles are needed. × 2 mm illed rice 0.5× 0.5 mm × 1 mm On an agarose gel assay, a portion

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