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Laboratory Math II: Solutions and Dilutions

Slide 1 Laboratory math II: Solutions and DilutionsPhilip Ryan, PhDPost-Doctoral FellowNational Cancer Institute, NIH Welcome to the National Institutes of Health, Office of Intramural Training & Education s Webinar on Laboratory math II: Solutions and Dilutions . This Webinar is intended to give a brief introduction into the mathematics of making Solutions commonly used in a research setting. While you may already make Solutions in the lab by following recipes, we hope this Webinar will help you understand the concepts involved so that you can calculate how to make any solution. Slide 2 Solutions A solution is a homogenous mixture of two or more substances. Can be in any form of matter: solid, liquid or gas Solutions are essential in most Laboratory -based biomedical research Examples: buffers, reaction mixtures, cell culture media, cell lysates, etc. A textbook definition of a solution is a homogenous mixture of two or more substances.

Normality is like molarity, but is used for ionic solutions to more accurately represent their ionic strength. A single molecule of an ionic compound may (when in solution) separate into individual charged particles. For example: NaCl in solution consists of positive charged sodium ions and negatively charged chloride ions. What is relevant is

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