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LECTURE 12 BIOREMEDIATION - MIT OpenCourseWare

LECTURE 12 BIOREMEDIATIONB ioremediationBioremediation is the use of microorganisms to destroy or immobilize waste materialsMicroorganisms include:Bacteria (aerobic and anaerobic)FungiActinomycetes (filamentous bacteria) BIOREMEDIATION mechanismMicroorganisms destroy organic contaminants in the course of using the chemicals for their own growth and reproductionOrganic chemicals provide:carbon, source of cell building material electrons, source of energyCells catalyze oxidation of organic chemicals (electron donors), causing transfer of electrons from organic chemicals to some electron acceptorElectron acceptorsElectron acceptors:In aerobic oxidation, acceptor is oxygenIn anaerobic, acceptor is (with decreasing efficiency):nitratemanganeseironsulfateM icro

May 11, 2004 · Example design calculations Moisture for biopile 375 yd3 gasoline-contaminated soil with otherwise low organic content Assume: porosity, n = 30% initial saturation, S = 20% Desired water content = 25 to 85%, use 60% Water needed = 375 yd3 (0.30) (0.6 – 0.2) = 45 yd3 = 1215 ft3 = 9,090 gallons

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