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LECTURE 5: LINKAGE AND GENETIC MAPPING Reading for …

Amacher LECTURE 5 (9/12/08) LECTURE 5: LINKAGE AND GENETIC MAPPINGR eading for this and previous LECTURE : Ch. 5, p 123-141 Problems for this and previous LECTURE : Ch. 5, solved problems I, II; #2 5, 7 9, 12, 14, 15,20, 21, 23, 24 and 27 Recombination results when crossing over during meiosis separates linked genesReciprocal exchanges between homologous chromosomes are the physical basis ofrecombination. using chromosomes that had cytologically visible abnormalities, Creighton andMcClintock working with maize, and Stern, working with Drosophila, showed thatrecombination depends upon the physical exchange of equal parts between maternal and paternalchromosomes during meiosis. Both groups followed chromosomes that were physically markedwith cytologically visible abnormalities, so that a maternally-derived homolog could be easilydistinguished from the paternal one.

Using chromosomes that had cytologically visible abnormalities, Creighton and McClintock working with maize, and Stern, working with Drosophila, showed that recombination depends upon the physical exchange of equal parts between maternal and paternal ... • Mapping reveals the relative order of genes, not the actual physical distance. ...

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