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Lentivirus and Lentiviral Vectors - University of Vermont

Lentivirus and Lentiviral Vectors Family: Retroviridae Genus: Lentivirus Enveloped Size: ~ 80 - 120 nm in diameter Genome: Two copies of positive-sense ssRNA inside a conical capsid Risk Group: 2 Lentivirus Characteristics Lentivirus (lente-, latin for slow ) is a group of retroviruses characterized for a long incubation period. They are classified into five serogroups according to the vertebrate hosts they infect: bovine, equine, feline, ovine/caprine and primate. Some examples of lentiviruses are Human (HIV), Simian (SIV) and Feline (FIV) Immunodeficiency Viruses. Lentiviruses can deliver large amounts of genetic information into the DNA of host cells and can integrate in both dividing and non-dividing cells.

engineering of second-generation vectors. In this system, the four accessory genes were eliminated leaving the gag and pol reading frames and the tat and rev genes. In general, lentiviral vectors with a wild-type 5’ LTR need the 2nd generation packaging system because they need tat for activation Transfer vector Transgene Ψ +

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