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M9 mineral medium - Helmholtz Zentrum München

M9 mineral mediumFor 1 liter m9 mineral medium add to 867 ml sterile water:100 mlM9 salt solution (10X) mM20 ml20% %1 ml1 M MgSO4 MgSO41 ml1 M mM1 mlbiotin (1 mg/ml)biotin1 g1 mlthiamin (1 mg/ml)thiamin1 g10 mltrace elements solution (100X)trace elements1 XStock solutionsM9 salt solution (10X) g/LKH2PO430 g/LNaCl5 g/LNH4Cl5 g/LDissolve the salts in 800 ml water and adjust the pH to with NaOH. Add water to afinal volume of 1 L and autoclave for 15 min at 121 Glucose20% (w/v) glucose200 g/LFor 500 ml stock solution add 100 g glucose to 440 ml water.

1.6 ml 0.1 M H3BO3 0.62 g/100 ml 8.1 µl 1 M MnCl2-4H2O 19.8 g/100 ml Arie Geerlof - Helmholtz Center Munich 15 May 2010. Created Date:

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