Transcription of MacConkey Agar
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MacConkey Agar
Please refer disclaimer Agar M081 BMacConkey Agar is recommended for selective isolation of Escherichia coli from pharmaceutical products and is in accordancewith harmonized methodology of BP. It is also recommended for selective isolation and differentiation of lactose fermenting and lactose non fermenting enteric bacteria. Composition**IngredientsGms / LitrePeptones (meat and casein) digest of after sterilization( at 25 C) **Formula adjusted, standardized to suit performance parametersDirectionsSuspend grams of dehydrated medium in 1000 ml purified/distilled water. Heat to boiling to dissolve the mediumcompletely. Sterilize by autoclaving at 15 lbs pressure (121 C) for 15 minutes validated cycle. AVOID to 45-50 C. Mix well before pouring into sterile Petri plates.
MacConkey Agar and Broth have been recommended for use in microbiological examination of foodstuffs (3) and for direct plating / inoculation of water samples for coliform counts (4). This medium is also accepted by the Standard Methods for …
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