Transcription of MGITTM Procedure Manual - FIND
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MGITTM Procedure Manual - FIND
Prepared for the Foundation for Innovative New Diagnostics MGIT TM. Procedure Manual For BACTEC MGIT 960 TB System (Also applicable for Manual MGIT). Mycobacteria Growth Indicator Tube (MGIT). Culture and Drug Susceptibility Demonstration Projects by Salman H. Siddiqi, (BD Fellow, Sparks, Maryland, USA ). and Sabine R sch-Gerdes, (Director, National Reference Center for Mycobacteria, Borstel, Germany). July 2006. TABLE OF CONTENTS. Section I: Principle of procedures A. Introduction B. Principle of the BACTEC MGIT 960 TB System 1. MGIT medium 2. Principle of detection and susceptibility testing Section II: Procedure for Primary Isolation A. Introduction B. Important Safety Precautions C. Specimen Handling 1. Collection 2. Transportation 3. Storage D. Digestion, Decontamination and Concentration 1. Sputum a. NaOH-NALC Procedure b. Other procedures c. Important points 2. Specimens other than sputum (extra-pulmonary). a. Pus and other mucopurulent specimens b.
50-60% of AFB culture-positive clinical specimens may fail to reveal AFB on smear made from the specimen. As a consequence, culture techniques play a key role in the diagnosis of mycobacterial disease. Egg-based media, such as Lowenstein-Jensen (LJ) or Ogawa have been used for cultivation of mycobacteria for several decades.
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