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Microarray Analysis - Information Technology Solutions

Microarray AnalysisThe BasicsThomas GirkeDecember 9, 2011 Microarray AnalysisSlide 1/42 TechnologyChallengesData AnalysisData DepositoriesR and BioConductorHomework AssignmentMicroarray AnalysisSlide 2/42 OutlineTechnologyChallengesData AnalysisData DepositoriesR and BioConductorHomework AssignmentMicroarray AnalysisTechnologySlide 3/42 Microarray and chip TechnologyDefinitionHybridization-based technique that allows simultaneousanalysis of thousands of samples on a solid ProfilingGene copy numberResequencingGenotypingSingle-nucle otide polymorphismDNA-protein interaction ( : chip -on- chip )Gene discovery ( : Tiling arrays)Identification of new cell technologiesProtein arraysCompound arraysMicroarray AnalysisTechnologySlide 4/42 Why Microarrays?

Microarray and Chip Technology De nition Hybridization-based technique that allows simultaneous analysis of thousands of samples on a solid substrate. Applications Transcriptional Pro ling Gene copy number Resequencing Genotyping Single-nucleotide polymorphism DNA-protein interaction (e.g.: ChIP-on-chip) Gene discovery (e.g.: Tiling arrays)

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