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Microbial Growth - IVCC

Microbial Growth Chapter 6. Biology 1009-Summer 2003. Johnson Microbial Growth n Refers to the number of cells, not the size of the cells n Requirements for Growth *Two general types: Physical: temp, pH, osmotic pressure Chemical: elements, including C, N, S, O, and P. Physical Requirements Temperature Psychrophile: cold loving Range 0C-20C. Mesophile: moderate temperature loving Range 20C-40C. Thermophile heat loving Range 40C-100C. 1. Physical Requirements (cont.). n pH. Most bacteria grow between pH Very few grow below pH 4. Some foods (pickles & sauerkraut). preserved from spoilage by acids made by bacterial fermentation Physical Requirements (cont.). n Osmotic Pressure Microbes obtain most of their nutrients in solution from surrounding water Many foods (honey, salted fish) preserved this way: high sugar or salt { } draws water out of bacterial cells, preventing their Growth Chemical Requirements n Carbon-50% of bacteria cell dry wt Microbes obtain from organic materials or CO2.

Microbial Growth nRefers to the number of cells, not the size of the cells nRequirements for growth *Two general types: ... nDue to the limiting factors in the environment Bacterial Growth Curve Direct Measurement of Microbial Growth 1) Plate counts -most used method/require serial dilutions

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