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Microbiome Analysis with QIIME2: A Hands-On Tutorial

Microbiome Analysis with qiime2 : A Hands-On Tutorial Amanda Birmingham C e nte r fo r C o m p u tatio n a l B io lo g y & B io in fo rm atics U n ive rs ity o f C a lifo rn ia at S a n D ie g o Software Selection Google 16S Analysis <program name> ; main contenders are Mothur Name: not an acronym (play on DOTUR, SONS). Philosophy: single piece of re-implemented software Top pro: easy to install Top con: re-implementations could be buggy Language: C++. Model: open-source License: GPL. Published: 2009. Developed: at Umichigan Software Selection Google 16S Analysis <program name> ; main contenders are QIIME.

Jun 21, 2018 · Earth Microbiome Project recommends 515f-806r primers, error-correcting barcodes • Not taking precautions to support amplicon sequencing Some Illumina machines require high PhiX, low cluster density • Selecting an inappropriate reference database E.g., Greengenes(16S) reference database when sequencing ITS

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