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Practical aspects of buffers Chemistry 201 NC State University Lecture 15 The everyday pH scale To review what pH means in practice, we consider the pH of everyday substances that we know from experience. Remember that [H+] = 10-pH. pH + pOH = 14 Therefore that [OH-] = 10pH-14. Two ways to make a buffer Add the acid and conjugate base to the solution in a defined proportion. Method 1 Method 2 Add a strong acid to the weak base (or vice versa) until the desired proportion [A-]/[HA] is obtained. Buffer strength The ratio [A-]/[HA] should be as close as possible 1:1, but the amounts may vary. To make a stronger buffer you simply need to increase the amount of each component. Let s investigate. Suppose we add 1 mL of 1 M HCl to 1 liter of solution. The final concentration of HCl is M.
Two ways to make a buffer Add the acid and conjugate base to the solution in a defined proportion. Method 1 Method 2 Add a strong acid to the weak base
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CHAPTER2 Acids, Bases and Salts, Acids, Bases, ACids, Bases and a -Base r, Base, Laboratory 24: Properties of Carboxylic Acids and, Properties of carboxylic acids and, DNA DNA = deoxyribonucleic acid, WEIR TYPE A DIAPHRAGM VALVES, Weir Type “A” Diaphragm Valves R, Hydroxyl Terminated Polybutadiene Resins and