Transcription of Nextera XT DNA Library Prep Kit Reference Guide (15031942)
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Nextera XT DNA Library Prep Kit Reference Guide (15031942)
NexteraXTDNAL ibraryPrepReferenceGuideDocument# document and its contents are proprietary to Illumina, Inc. and its affiliates ("Illumina"), and are intended solely forthe contractual use of its customer in connection with the use of the product(s) described herein and for no otherpurpose. This document and its contents shall not be used or distributed for any other purpose and/or otherwisecommunicated, disclosed, or reproduced in any way whatsoever without the prior written consent of Illumina. Illuminadoes not convey any license under its patent, trademark, copyright, or common-law rights nor similar rights of any thirdparties by this instructions in this document must be strictly and explicitly followed by qualified and properly trained personnel inorder to ensure the proper and safe use of the product(s) described herein.
Assess DNA Purity UV absorbanceis a common methodusedfor assessing the purity of a DNA sample.The ratio of absorbance at 260 nm to absorbanceat 280 nm provides an indication of sample purity.This protocol is optimizedfor DNA with 260/280 absorbanceratio values of 1.8–2.0,which indicates a pure DNA sample.
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