Transcription of NextSeq System Denature and Dilute Libraries Guide …
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NextSeq System Denature and Dilute Libraries Guide …
NextSeqSystemDenature andDilute LibrariesGuideOverview3 Consumables and Equipment4 ProtocolA: StandardNormalizationMethod4 ProtocolB: Bead-BasedNormalizationMethod6 ProtocolC: AmpliSeqfor IlluminaPanelsNormalizationMethod7 ProtocolD: AmpliSeqLibraryEqualizerfor Illumina NormalizationMethod9 ProtocolE: TruSight Tumor170 LibraryDenaturationand DilutionMethod11 Protocol F: TruSight Oncology500 LibraryDenaturationand DilutionMethod12 Denature andDilute PhiXControl (ProtocolsA D)13 Denature andDilute PhiXControl (ProtocolE)15 Denature andDilute PhiXControl (Protocol F)16 Next Steps18 PreparePhiX for a TroubleshootingRun18 RevisionHistory20 TechnicalAssistance21 Document#15048776v09 December2018 ILLUMINAPROPRIETARYFor Research UseOnly. Not for use in diagnostic document and its contents are proprietary to Illumina, Inc. and its affiliates ("Illumina"), and are intended solely forthe contractual use of its customer in connection with the use of the product(s) described herein and for no otherpurpose.
NextSeq System Denature and Dilute Libraries Guide (15048776) Author: Illumina Subject: Instructions for denaturing and diluting libraries before sequencing on the NextSeq 500 and NextSeq 550 systems.
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