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腸内細菌科菌群の検出試験法(増菌培養法) NIHSJ-15-ST4:2011

1 ( ) NIHSJ-15-ST4:2011 (Enterobacteriaceae) (VRBG) ISO 21528-1:2004 25 g 225 ml 37 C 18 2 1 ml EE 10 ml 37 C 24 2 VRBG 37 C 24 2 1) (Buffered Peptone Water; BPW) 121 C 15 * g NaCl g Na2 HPO4 12H2O g KH2PO4 g 1,000 ml pH * 2) (Buffered Brilliant Green Bile Glucose Broth EE ) 10 ml 30 25 C pH * g g Na2 HPO4 g KH2PO4 g g g 1,000 ml * 3) (Violet Red Bile Glucose agar VRBG )

2 調整する。本培地を滅菌してはならない。 * 獣肉ペプトンなど ** 寒天のゲル強度による 4) 普通寒天培地(Nutrient Agar)

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Transcription of 腸内細菌科菌群の検出試験法(増菌培養法) NIHSJ-15-ST4:2011

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