Transcription of NMR of Proteins
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NMR of Proteins
NMR of ProteinsNMR of ProteinsDetermining Protein Structures by NMRD etermining Protein Structures by NMR the process of determining a solution structure by NMR is one of measuring many (hundreds/thousands) of short proton- proton distances and angles, and restraining the protein structure with these computationallyH H ..}d1H NMR Spectra of Proteins1H NMR Spectra of Proteins 1D, 1H NMR spectra of even small Proteins are impossible to interpret in any comprehensive manner -normally, only gross statements about secondary structure, tertiary structure, etc.
-enhancement depends on 1/r6, where r is the internuclear distance-thus, the effect is limited to distances of approximately 5Å or less • This provides a means to determine if any two protons in a protein are < 5Å apart • The basic experiment used for proteins is called a NOESY 90 90 90 2D NOESY t 1 τ m t 2 The Nuclear Overhauser Effect
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