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The Handbook of Metabonomics and MetabolomicsJohn C. Lindon, Jeremy K. Nicholson and Elaine Holmes (Editors) 2007 Published by Elsevier 3 NMR Spectroscopy Techniques for Applicationto MetabonomicsAlfred Ross, Goetz Schlotterbeck, Frank Dieterle, and Hans SennPharma Research, F. Hoffman La-Roche AG, Basel IntroductionSince its discovery in the 1940s,NuclearMagneticResonance (NMR) Spectroscopyhas become a powerful, interdisciplinary method. A brief historical review wouldreveal as many as nine Nobel Prize laureates since the time when Isador I. Rabi devel-oped resonance methods for recording the magnetic properties of atomic nuclei andwas awarded the Nobel prize in physics (1944). The NMR phenomenon was soon laterdemonstrated for protons. After years of continuous development, Fourier Transform(FT) NMR entered the scene in the 1960s, followed by the evaluation of ,three-dimensional(3D) structure elucidation of proteins at atomic resolution in aqueous environment wasdeveloped [1].
56 NMR Spectroscopy Techniques play in areas of pharmacology, toxicology and the investigations of inborn errors of metabolism [4–6]. Since these early applications, 1H NMR of biofluids and cell extracts including high-resolution magic angle spinning (HR-MAS) NMR of soft tissues have been
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