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Notes CYTOLOGY : STAINING METHODS

MODULE CYTOLOGY : STAINING METHODS Histology and CYTOLOGY 25. Notes CYTOLOGY : STAINING . METHODS . INTRODUCTION. Consistency and reliability are most important in cytological interpretation. Cytologists rely heavily on the quality and appearance of the stain. The Papanicolaou stain is recommended for the STAINING of alcohol fixed CYTOLOGY slides. Romanowsky stains may also be used for wet fixed slides, but are primarily applied to air-dried smears. Special stains are used as per requirements: Modified Ziehl Neelson (for acid fast bacilli), Gram STAINING (Bacteria), Mucicarmine (mucins), PAS (for glycogen, fungal wall, lipofuscin, etc), Oil red O (lipids), Perl's Prussian blue (iron), modified Fouchet's test (bilirubin), etc.

zdescribe the principle of cytology stains zexplain the methods of staining cytology specimens. 25.2 STAINING IN CYTOLOGY The universal stain for cytological preparations is the Papanicolaou stain. Harris’ hematoxylin is the optimum nuclear stain and the combination of …

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