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Nuclear Speckles - Lamond Lab

Nuclear SpecklesDavid L. Spector1and Angus I. Lamond21 Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, New York 117242 Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee,MSI/WTB/JBC Complex Dow Street, Dundee DD1 5EH, United Speckles , also known as interchromatin granule clusters, are Nuclear domainsenriched in pre-mRNA splicing factors, located in the interchromatin regions of the nucleo-plasm of mammalian cells. When observed by immunofluorescence microscopy, theyusually appear as 20 50 irregularly shaped structures that vary in size.

(Herrmann et al. 2001). In addition, a signifi-cant overlap between cyclin T1 and nuclear speckles was observed. However, although Cdk9 was present in the vicinity of nuclear speckles,

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