Transcription of One-step real-time duplex reverse transcription …
{{id}} {{{paragraph}}}
Example: confidence
One-step real-time duplex reverse transcription …
508 BioTechniques Vol. 36, No. 3 (2004)RNA TECHNOLOGIESVol. 36, No. 3 (2004) BioTechniques 509 INTRODUCTIONOf all methods used to quantify mRNA, real - time reverse transcrip-tion PCR (RT-PCR) is considered the most sensitive and accurate one (1). In addition to its wide use in labora-tory research, real - time RT-PCR has the potential for broad application in the diagnoses of functional defects in disease. The absolute quantification of mRNA is generally impractical and unnecessary because constantly transcribed housekeeping genes ef-fectively serve as internal standards for the relative quantification of the transcripts of genes of interest (1 5).
508 BioTechniques Vol. 36, No. 3 (2004)
Loading..
Tags:
Information
Domain:
Source:
Link to this page:
Please notify us if you found a problem with this document: